primary antibodies against cc10  (Millipore)

Journal: Redox Biology

Article Title: Aryl hydrocarbon receptor in club cells drives Th17-mediated lung injury following inhalation exposure to environmentally persistent free radicals

doi: 10.1016/j.redox.2026.104105

Figure Lengend Snippet: Generation and validation of club cell-specific AHR knockout mice ( Ahr ΔCC). (a) Schematic of breeding strategy to generate Ahr ΔCC mice by crossing Ahr fl/fl mice with Scgb1a1-CreER TM mice, followed by tamoxifen induction. (b) Immunofluorescence staining showing club cell marker CC10 (green), AHR (red) and DAPI (blue) in lung sections of Cre-negative Ahr fl/fl LM control (top panel) and Ahr ΔCC (bottom panel) mice. (c) Representative flow cytometry plots of lung epithelial cells gated as CD45 − CD31 − EpCAM + CC10 + cells isolated from lungs of LM (left) or Ahr ΔCC (center) mice and Fluorescence-minus-one (FMO) control for AHR staining (right). (d) Quantification of the percentage of AHR + CC10 + cells in the lungs of LM (white bar) and Ahr ΔCC (gray bar) mice. Data represent mean ± SEM, n = 3-4 mice per group. Statistical significance was determined using Student's t-test; ∗p < 0.05.

Article Snippet: After washing, slides were incubated overnight at room temperature with a second primary antibody against CC10 (Proteintech, Rosemont, IL; catalog# 10490-1-AP), followed by an Alexa Fluor Plus 488-conjugated goat anti-rabbit secondary antibody (Invitrogen, Waltham, MA; catalog# A32731).

Techniques: Biomarker Discovery, Knock-Out, Immunofluorescence, Staining, Marker, Control, Flow Cytometry, Isolation, Fluorescence

Journal: International Journal of Nanomedicine

Article Title: A Novel Airway-Organoid Model Based on a Nano-Self-Assembling Peptide: Construction and Application in Adenovirus Infection Studies

doi: 10.2147/IJN.S413743

Figure Lengend Snippet: Cells Marker Proteins Associated with Airway Tissue

Article Snippet: Primary antibodies against CC10 (catalog No. 49757), VIM (catalog No. 21488), CD31 (catalog No. 29555), AQP5 (catalog No. 29573), SPC (catalog No. C32459) and KRT8 (catalog No. 38010) and secondary antibodies against AF488-labeled goat anti-mouse IgG (catalog No. L3016) and AF647-labeled goat anti-rabbit IgG (catalog No. L3038) were purchased from Signalway Antibody LLC (USA).

Techniques: Marker, Fluorescence

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Proteomic Analysis Reveals the Deregulation of Inflammation-Related Proteins in Acupuncture-Treated Rats with Asthma Onset

doi: 10.1155/2012/850512

Figure Lengend Snippet: Protein expression profiles in lung tissues. (a) A representative 2DE gel image of proteins isolated from the AA group. The numerically labeled spots indicate the differentially expressed protein spots among the NC, AS, and AA groups. The numbers correspond to the spot identification numbers listed in . The molecular weight standards and pH range are shown at the left and bottom of the gels, respectively. (b) Differential expression profiles of five inflammation-related proteins regulated by acupuncture: CC10, S100A8, RAGE* (which is actually sRAGE, ), RhoGDI2, and ANXA5. The cropped images of 2DE gels were symmetrically boxed, and the arrows on the images indicate the relative positions of the protein spots. (c) Quantitative analysis of the five inflammation-related proteins regulated by acupuncture. Each spot volume was quantified from the intensity of the spots using PDQuest software. The bars represent the mean ± SEM of triplicate 2DE gels. # P < 0.05, ## P < 0.01 when comparing the AS group with the NC group. * P < 0.05, ** P < 0.01 when comparing the AA group with the AS group.

Article Snippet: The blocked membranes were incubated overnight at 4°C with specific primary antibodies against CC10 (1 : 5000, Millipore 07–623), RhoGDI2 (1 : 2000, Abcam ab14230), S100A8 (1 : 3000, Santa Cruz sc-8113), sRAGE (1 : 5000, R&D AF1616), and β -actin (1 : 5000, Abcam) and were washed and incubated with the appropriate horseradish-peroxidase- (HRP-) conjugated secondary antibodies for three hours at room temperature.

Techniques: Expressing, Isolation, Labeling, Molecular Weight, Quantitative Proteomics, Software

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Proteomic Analysis Reveals the Deregulation of Inflammation-Related Proteins in Acupuncture-Treated Rats with Asthma Onset

doi: 10.1155/2012/850512

Figure Lengend Snippet: Validation of the expression profiles of five inflammation-related proteins by western blot analysis. (a) A representative western blot visualizing the expression levels of CC10, S100A8, and RhoGDI2. β -actin was used to demonstrate equal loading. (a) Western blot analysis using the anti-rat RAGE extracellular domain monoclonal goat antibody demonstrates that the lung homogenate contains three bands that were approximately 48, 50, and 55 kD in size; the 48-kD band corresponds to sRAGE and the 55 and 50 kD bands correspond to full-length RAGE. (b) The densitometric quantification of individual proteins is expressed as the fold change compared to β -actin. Each bar represents the mean ± SEM of triplicate experiments, and similar results were observed in all experiments. # P < 0.05, ## P < 0.01 when comparing the AS group with the NC group; * P < 0.05, ** P < 0.01 when comparing the AA group with the AS group.

Article Snippet: The blocked membranes were incubated overnight at 4°C with specific primary antibodies against CC10 (1 : 5000, Millipore 07–623), RhoGDI2 (1 : 2000, Abcam ab14230), S100A8 (1 : 3000, Santa Cruz sc-8113), sRAGE (1 : 5000, R&D AF1616), and β -actin (1 : 5000, Abcam) and were washed and incubated with the appropriate horseradish-peroxidase- (HRP-) conjugated secondary antibodies for three hours at room temperature.

Techniques: Biomarker Discovery, Expressing, Western Blot

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Proteomic Analysis Reveals the Deregulation of Inflammation-Related Proteins in Acupuncture-Treated Rats with Asthma Onset

doi: 10.1155/2012/850512

Figure Lengend Snippet: A possible inflammatory signaling pathway that results from the functional associations of the identified inflammation-related proteins (marked with an underline), which is regulated by acupuncture in asthma. Acupuncture can downregulate the proteins S100A8, S100A9, RAGE, and RhoGDI2 and upregulate the expression of CC10 and sRAGE. This pathway may explain the anti-inflammatory effect of acupuncture in asthma. Rho inact, inactivated Rho GTPase; Rho act, activated Rho GTPase; RhoGEF, Rho-specific guanine nucleotide exchange factors; GAP, GTPase-activating proteins; NF- κ B, nuclear factor- κ B; AP1, activator protein-1.

Article Snippet: The blocked membranes were incubated overnight at 4°C with specific primary antibodies against CC10 (1 : 5000, Millipore 07–623), RhoGDI2 (1 : 2000, Abcam ab14230), S100A8 (1 : 3000, Santa Cruz sc-8113), sRAGE (1 : 5000, R&D AF1616), and β -actin (1 : 5000, Abcam) and were washed and incubated with the appropriate horseradish-peroxidase- (HRP-) conjugated secondary antibodies for three hours at room temperature.

Techniques: Functional Assay, Expressing